Airplane! is a 1980 American satirical comedy film directed and written by David Zucker, Jim Abrahams, and Jerry Zucker and released by Paramount Pictures. It stars Robert Hays and Julie Hagerty and features Leslie Nielsen, Robert Stack, Lloyd Bridges, Peter Graves, Kareem Abdul-Jabbar and Lorna Patterson. The film is a spoof of the disaster film genre.

In "Death Is a Bitch", the varied passengers on the plane, nuns, Girl Scouts, etc., are reminiscent of the film.

Peter slaps Lois in the bathroom, yelling “Get a hold of yourself,” in "He's Too Sexy for His Fat".

Dr. Rumack, a character from the film, is portrayed by Leslie Nielsen. Rumack appears in Blue Harvest telling Han Solo (Peter) "I just want to tell you both good luck, we're all counting on you". C-3PO, played by Glenn Quagmire shakes Leia while saying "...and Leia's getting laaaarger."

Many characters, including Herbert, Evil Monkey, Elmer Hartman, Mayor West, and Kool-Aid Man line up to shake Stewie in "Prick Up Your Ears".

"Padre de Familia" translates its title into Spanish as 'Incredible Flying Joke Bus'.

In "Peter's Daughter", The scene where Stewie and Brian are renovating the run-down house, and becoming confused with the requirement to say "over" when using the walkie-talkie may be a distant reference to the sequence in the movie, where confusion is caused by the name of Captain Oveur, pronounced "over".

When Brian starts to lie about how he likes Loretta in "Love, Blactually", Brian's nose starts to grow like Pinocchio's nose does. Brian also repeats the line, "free to pursue a life of religious fulfillment", which is a line uttered by Dr. Rumack.

"Airport '07" has many references to the film.

When New Brian walks into the kitchen just before the "I Like Farts" song, Peter says, "Oh, you have a guitar!" just like the mother of the sick girl does in the film Airplane!.

Jerome and Quagmire quote Jive in "Jerome Is the New Black".

Peter beats up a bunch of annoying people while holding in his poop in "Mom's the Word" as a parody of a scene where Rex Kramer (Robert Stack) beats up a mob of religious recruiters while calmly walking through the airport.