The Agatha Christie detective novel And Then There Were None is credited as the primary reference in the DVD commentary.
Opening title sequence resembles the one from Stanley Kubrick's The Shining.
Stephanie starts bleeding after Brian pops the cork on a champagne bottle, similar to a scene in Indiana Jones and the Temple of Doom.
Seamus' story of how he was made is a parody of Pinocchio.
When James Woods turns up dead, Peter says "criss-cross!", a reference to the Alfred Hitchcock movie Strangers on a Train.
When dressed in armor, Peter says, "Go-go Gadget Skis!" A reference to the TV series Inspector Gadget.
When the group is carrying Joe up the stairs, Peter jokingly hums "Hava Nagila". This is a parody of the Jewish tradition of when at bar or bat mitzvah ceremonies or wedding receptions, those being honored get lifted on a chair to this music.
Carl makes references to the films Labyrinth, No Way Out, and The Great Outdoors. In the DVD extended edition, he also makes references to Hard Rain and Days of Thunder.
When the remaining group sees Tom Tucker as the murderer, Peter says "Tell that to Mike Judge!", a reference to the quote "Tell that to the judge!" and the creator of King of the Hill.
When Dr. Hartman pretends to attack Seamus, he imitates the music from Psycho.