Anna Lee Quagmire, nicknamed Anal, is Glenn Quagmire's daughter that he didn't know about. She was left at her dad's doorstep by her mother, one of Glenn's one-night-stands. Her mother left her in a basket during a garage sale he held in "Quagmire's Baby". He couldn't take care of her because she was messing up his sexual relationships, so he gives her up for adoption. He later realizes that he made a big mistake. He finds the family that adopted her and realizes that she is happy where she is. Like her father, she says "Giggity".

She is voiced by Mae Whitman.

Relatives

• Glenn Quagmire (Father)
• Joan Quagmire (Step-Mother) Deceased
• Unnamed mother
• Dan/Ida Quagmire (Paternal grandfather)
• Mrs. Quagmire (Paternal grandmother)
• Brenda Quagmire (Paternal aunt)
• Gary Quagmire (Paternal uncle)
• Abby (Cousin)
• Quagmire's kids (Brothers)