Disneyland Park, commonly known as Disneyland, is the first of two theme parks built at the Disneyland Resort in Anaheim, California, opened on July 17, 1955. Spanning 160 acres and the only theme park to be designed and built under the direct supervision of Walt Disney, it is dedicated to fairy tales and Disney characters.

In "Brian Does Hollywood", Brian has a job at a carwash and, hoping to become a director, presents to each of his customers a script of his own screenplay. Former Disney CEO Michael Eisner pulls up to the carwash, but ignores Brian's offer of the script and simply pulls out a Mickey Mouse hat with "Brian" on it. He then tells Brian to come to Disneyland, but just before driving off, he shouts, "Bring money!"

The family rides the Mad Tea Party at Disneyland in the "No Bones About It" segment of "Family Guy Viewer Mail No. 1" and Peter flies out. Afterwards, he was mistaken to be a towel by Michael Eisner.

During the afterparty in Stewie Griffin: The Untold Story, Peter explains about having a series of part-time jobs after Family Guy was canceled including as Mickey Mouse at Disneyland.

When Brian, Mort Goldman and Stewie arrive to Berlin in "Road to Germany", the city is called "The Happiest Place on Earth", which is the nickname for Disneyland.

A photo of Billy Corgan riding the Big Thunder Mountain roller coaster from a trip to Disneyland in 2015 appears in "Saturated Fat Guy".

Stewie refuses a request to duck down out of a family photo on a ride intended to be Splash Mountain at Disneyland in "Switch the Flip". To further spite them, he stands up but ends up decapitated with his head in another family's log.