There are two instances of Stewie's potential homosexuality: Stewie points out he is not all in Brian's face with 'his heterosexuality' to which Brian replies: 'Ah, no you are not.' Later, the brochure for straight camp turns out to have been in Stewie's possession for quite some time before Peter was injected with the gay gene.
This is the fourth instance of the Cleveland's Bathtub Gag, although this one was purely an accident. Peter was trying to back his car and the horse wouldn't move and when it did, the car had enough gas so that it crashed into Cleveland's house.
The horse race sign reads 'TODAY QUAHOG DERBY - TOMORROW TERRENCE TRENT D'ERBY. This is the second Terrance Trent D'Arby reference in season 7 along with "Road to Germany".
Peter mentions one of past jobs was being Matt Damon's neck. The scene then cuts to Damon with Peter's face on his neck annoying him with his role in Good Will Hunting, calling him "an educated Boston street thug" and his friend Ben Affleck's marriage to Jennifer Garner.
When Peter first returns home after receiving the gay gene, his voice sounds very similar to Roger from American Dad. This is because Seth MacFarlane voices both characters.
The Parents Television Council, a watchdog organization and significant critic of Family Guy, filed an FCC complaint against FOX about this episode and specifically cited the 'eleven-way' gay orgy, the (alleged) consumption of horse semen by Stewie and Peter thinking Lois performs a sexual act on him when his horse is licking his butt.