The main plot of this episode is a parody of the film Tootsie and incorporates many references from that film, including the episode title, from a lyric in the film's title song by Stephen Bishop, "Go, Tootsie, Go!". Stewie's "argument" with his alter-ego is a parody of a similar scene originally having been performed in Tootsie.
The Seinfeld theme is played after Meg catches Anthony cheating on her with her mother Lois.
Parts of the American version of Jolly Farm Revue parody Sesame Street.
The name of Stewie's female alter ego, "Karina Smirnoff," is the name of a professional dancer best known for her appearances on Dancing with the Stars.
Stewie and Jolly Farm make a reference to PanAm flight 103; A Boeing 747-100 carrying 243 passengers and 18 crew which exploded over Lockerbie, Scotland by a terrorist bomb on December 21, 1988; killing all 243 passengers and 16 crew members. The debris from the 747 fell on the town, killing 11 people on the ground, bringing the death toll to 270.