Mrs. Wilson is a teacher at Martin Mull Elementary School.

She was Peter Griffin's third grade teacher when he was a child. Griffin and his friends called her "Mrs. Kill-son" because she had an abortion. When Griffin needs to pass the third grade in order to get a promotion in "Tales of a Third Grade Nothing", she becomes his teacher once again. One of her other students is Glenn Quagmire's illegitimate children. She passes Peter after he wins a spelling bee for his school, facing Omar, another child in her class.

Mrs. Wilson is voiced by Debbie Reynolds.