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Siegfried & Roy are a German-American duo of former contemporary magicians and entertainers who became known for their appearances with white lions and white tigers.
In "Meg Stinks!", Stewie compares a half-feral Brian to Mantecore, a tiger that attacked Roy Horn in 2003. A cutaway shows Mantecore stopping by Horne's hospital room, concerned about getting a test, implied to be an AIDs test.